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Home » Growing » Plant Propagation via Tissue Culture

Plant Propagation via Tissue Culture

Updated : November 7, 2014

13 Comments

Plant Propagation via Tissue Culture is the most modern and scientific method of propagating new plants.

Plant propagation via tissue culture is a form of micro propagation where tiny pieces of plant tissue are used to produce exact clones of the plant from which the tissue originated.  In the nursery business many plants are not grown from seed as most people think because the seeds collected from the desired plant will not produce an exact clone of that particular plant.

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For instance, if you collect seeds from a Pink Dogwood tree and sow those seeds, the seedlings that you grow will be white flowering dogwoods, not pink.  The chances of getting a Pink Dogwood tree from seed are probably one in a million I’d guess.

If that’s the case, then where did the first Pink Dogwood tree come from? From seed!  The first Pink Dogwood tree was that one in a million plant that nurserymen dream of and many, many years ago when the first Pink Dogwood tree was spotted somebody was astute enough to make sure that particular tree was propagated via vegetative means so it could be reproduced many, many times over the years.

Then years later somebody found another dogwood seedling that flowered pink but this time the flowers were a much deeper pink and that particular tree was propagated via vegetative means so us plant lovers of today can enjoy Pink Dogwoods with deep pink blooms like the popular Cherokee Chief Pink Dogwood Tree.

In short, what I am trying to say is that most of the beautiful landscape plants that we enjoy today cannot be grown from seed, they have to be grown from a cutting, a single bud via budding, or a scion via grafting.  All of these techniques involve collecting pieces of the parent plant and finding ways to either get the pieces to make their own roots, or attach those pieces to a rootstock that already has roots via budding or grafting.

But what’s important to understand is that in order to produce an exact clone of a plant must first remove tissue from that plant to make a new plant.  Propagation by cuttings, budding, or grafting work well and those three techniques have been in use for hundreds of years.  However, you can only collect so many cuttings, buds, or scions from from a single plant.

Then along comes a scientific process known as tissue culture.

With tissue culture you can collect a very small amount of tissue from the parent plant and using scientific strategies performed in an actual laboratory, you can actually trick that tissue into multiplying itself exponentially creating thousands and thousands of new plants at one time.

The process makes me think of splitting the atom.  I’m about the farthest thing you’ll ever see from a scientist so I’m not sure that I can even explain this and make you understand.  Basically it’s like making test tube babies by the tens of thousands.

Of course the tiny plants that you produce in a tissue culture lab are really, really tiny and they have to be cared for in a very sterile environment until they are strong enough to enter the real world as I say!  I think of these tiny plants much like you would a really, really premature baby.

Everything about tissue culture is very, very scientific and it involves complicated chemical compounds etc.  When done correctly a tissue culture lab can crank out plants faster than the industry could ever find a place for them.  In many cases it can also crank out plants that are superior to plants that are grown by more traditional means.  Plants that are more disease resistant, have better branching etc.

But then again, because you are creating exact clones, you might crank out a bunch of plants that are all vulnerable to some kind of a virus that could wipe them all out.

What I do know is that certain kinds of plants are commonly produced in tissue culture labs today, but most nurseries still rely on the old fashioned methods of in house plant propagation.

Years ago one of our more progressive local growers invested a significant amount of money to build a tissue culture lab at their nursery.  After about three frustrating years of trying to make that lab do what they expected it today the closed it up and walked away from it.  And this nursery is one of the most progressive plant propagation nurseries in this area, and they could not make it work for them without all kinds of issues and frustrations.

In other words, tissue culture is best performed by scientists.

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Comments

  1. Alissa Shannon says

    July 19, 2016 at 5:08 pm

    Hey Mike, thank you so much for the info you share.
    I have a question about propagation methods. How do you know which is the best method to produce the strongest plant? For instance I read somewhere that people don’t do cuttings of cherry trees because, though it would work, the root system is much stronger if it is started from seed. So, it’s better to have root stock started from seed and graft the cultivar you want onto that. Is that true? I read the same for Actinidia Arguta vines (hardy kiwi vines). I’m wanting to get into propagating edible plants, but this one question is the one that keeps stumping me and I haven’t had any success finding the answer. Thank you for your time.

    Reply
    • Mike says

      July 19, 2016 at 7:08 pm

      Alissa,

      It’s a bit tricky to know what is best for each plant. That comes from experience and communicating with other growers, http://backyardgrowers.com/join. But to answer your question most fruit trees are budded or grafted onto a root stock. Other plants like raspberries, strawberries and blueberries are different. Blueberries can be done from cuttings, raspberry as well, layered or root cuttings. Strawberries layered or divided as well.

      Reply
  2. Melody Arnett says

    June 23, 2014 at 5:40 pm

    The Princess Tree is tissue prop?

    The real name is Paulownia and the spring blooms look like foxglove and they are the fastest growing trees in the world? I have several trees growing from the surface root of the mother tree and I don’t want to kill them. Each time I try to remove and replant them, they die! PLEASE tell me how can I successfully replant these?

    Thank you!

    Reply
    • Mike says

      June 24, 2014 at 7:01 am

      Melody,

      You have to remove them when the plant is dormant because you are severing roots. Chances are they will grow from cuttings, form their own roots, then you’d have more success. http://www.freeplants.com/homemade-plant-propagation.htm

      Reply
  3. Heather says

    February 3, 2013 at 11:37 am

    Thanks! I enjoyed that educational message!

    Reply
  4. patricia donovan says

    February 3, 2013 at 5:15 am

    Mike I live in South America- Argentina-
    when is the best time to do the crafting in maple trees?

    Reply
    • Mike says

      February 3, 2013 at 10:19 am

      Patricia, the ideal time to graft is mid to late winter. Once the scion wood buds start to swell before being removed from the parent plant it’s probably too late. Here in the states, Ohio, we graft late January, through most of February. Our plants typical leaf out in full around mid April. I hope this helps.

      Reply
    • al says

      February 9, 2013 at 4:03 pm

      before they break dormancy

      Reply
  5. Bob Donker says

    February 2, 2013 at 10:15 pm

    Most of this is all new to me, as a beginner, but more then willing to try.
    We just invested in a 5 acre wooded property and with Mikes help and suggestions, we hope to plant the right things in the right place.
    Thanks Mike.

    Reply
  6. Rick says

    February 2, 2013 at 9:08 pm

    Back in the 70’s there was a phrase: “Appropriate Technology”. The idea was to use the methods and tools that made sense for the situation. If you were a poor farmer it didn’t make sense to use materials that cost more than you could afford… more than your profits. But many fell victim to slick advertising and bought special seeds and chemicals that drove them into heavy debt and bankruptcy while ruining their soils… it even cost many their traditional family farms. Sometimes the best way isn’t the most ‘advanced’ way. People have been growing plants and food for thousands of years – ORGANICALLY – long before the advent of artificial chemicals and fertilizers that kill soil microorganisms and cause health problems like cancer. I hope everyone will give this some thought before they invest in inappropriate exotic, expensive, and toxic methods. Look at the long term impacts, not just the short term results.

    Reply
  7. Bill Thomassen says

    February 2, 2013 at 12:26 pm

    Is this how they propagate orchids?
    Anxiously awaiting the article!

    Reply
    • Mike says

      February 2, 2013 at 6:42 pm

      Bill, I think it’s safe to say that Orchids have been around a lot longer than tissue culture, but I’m sure some are done that way now.

      Reply
    • al says

      February 9, 2013 at 4:03 pm

      yes
      anything
      stertile is the key word

      Reply

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